In essence, BD CBA is equivalent to running up to 30 ELISAs at once using flow cytometry. Turn on the computer and log in with your UCF info. Historically, laboratories have relied on heterotrophic plate counts (HPCs) to monitor water quality, but this method is unreliable and time intensive. SYBR® is a registered trademark of Life Technologies Corporation. Calcium levels increased immediately after treatment with the calcium ionophore A23187 (left plot), but not after treatment with DMSO control (right). T-cell phenotyping data analyzed on a BD Accuri C6, Identification and gating of five peripheral blood cell populations, Differentiating naïve and memory T cells using the kit and software template, Distinguishing Tregs with CD127 using the Treg cocktail and template, Identifying three lymphocyte populations using two detectors. Despite its compact size, the optical system demonstrates extraordinary fluorescence sensitivity. The level of phosphorylation can easily be quantified as a function of median fluorescence intensity (MFI). Figure 3-3. The direct counts correlate highly with counting beads (r. Using the BD Pharmingen Human Th1/Th2/Th17 Phenotyping Kit (Cat. Changing the Workstation Computer Settings for Windows ® 7. Allow. Find out how this powerful platform, combined with BD kits and templates, will give you more insight and easier workflow. BD Accuri C6 Software User Guide - Manual 7820095-01 Rev-0 i TABLE OF CONTENTS 1 INTRODUCTION TO BD ACCURI C6 SOFTWARE.....1 1.1 Starting BD Accuri C6 Software .....1 1.2 BD Accuri C6 Software Workspace .....2 1.3 Opening a New BD Accuri C6 Software Workspace .....2 1.4 Exiting BD Accuri C6 Software.....3 1.5 Using the Example BD Accuri C6 Software File.....3 2 VALIDATING THE … These characteristics allow flow cytometry to be used in diverse microbiological applications, such as measurement of gene expression, monitoring bacterial and yeast fermentations, recombinant protein production in bacteria, and food processing. The following sections and resources illustrate the rich data you can generate by using BD Accuri systems for immunology and stem cell applications. 7. Contact Sales. BD Accuri C6 Plus software features an intuitive user interface. Press the Power button on the BD Accuri C6 instrument face panel. The white paper presents sample data and guidance for viable cell concentrations in cultured cell lines, immune cell concentrations in human peripheral blood, and platelet counts in whole, unlysed human blood. For walkaway convenience, the optional BD CSampler™ Plus accessory offers reliable and easy-to-use automation. The BD CSampler Plus adds a minimal footprint to the BD Accuri C6 Plus, about three feet square for the pair, keeping your benchtop free for other uses. 6) as far as I know, BD now offers of purchasing the new BD C6+ cytometer with a discount of ~11.000 Eur if you give back the old C6. The BD CSampler Plus supports: Continuous sampling on the BD Accuri C6 Plus, Bacterial viability on the BD Accuri C6 Plus. The cells were then fixed, lysed and permeabilized using the BD Pharmingen™ Human Transcription Factor Buffer Set (Cat. The system is equipped with a blue laser and a red laser, This option significantly expands the fluorochrome combinations you can analyze. Deciphering the cell surface proteome enables researchers to define strategies for the analysis and isolation of targeted cells from heterogeneous populations for functional studies, drug screening, and in vivo animal studies. Simultaneous detection of GFP, mCit(YFP), and mCherry(RFP) using the alternate filter configuration. The optional accessory offers reliable, easy-to-use automation while adding minimal footprint. In the first two interviews below, researchers describe how they use BD Accuri systems to detect foodborne pathogens and monitor fermentations. BD Accuri™ C6 Plus is for Research Use Only. 9. The graph depicts the live and non-viable cell concentrations as a function of time. BD Accuri™ C6 Plus Flow Cytometer Technical Specifications The BD Accuri™ C6 Plus flow cytometer is easy to use, simple to maintain, and affordable. BD Accuri ™ BD Alaris ™ ... Overview Specifications Resources. Jurkat cells (human T-cell leukemia; ATCC TIB-152) were treated with 0.025% DMSO vehicle control for 5 hours (red), 5 μM camptothecin for 5 hours to induce apoptosis (blue), or 50 μM FCCP mitochondrial uncoupler for 20 minutes (green). BD FACSVia™ System is CE marked in compliance with the European In Vitro Diagnostic Medical Device Directive 98/79/EC. StatLock™ Stabilization Devices are a more effective alternative to tape in helping improve clinical outcomes, quality of care and economic efficiencies. Current extended warranties will not be affected and will be supported until they expire. Search results for accuri optical filter on BD Biosciences-US Student groups at California State University, Northridge, cultured CEM-C7-14 leukemia cells at a designated cell density. Apr 9th, 2020 Bioprocess Monitoring With The BD Accuri™ C6 Flow Cytometer Bioprocess Monitoring With The BD Accuri™ C6 Flow … These characteristics can make them difficult to process through a flow cytometer’s fluidics system, even when pond water is filtered before acquisition. Flow Cytometry Application in CRISPR-Cas Systems and Molecular Biology Assays, Introducing the New BD Accuri™ C6 Plus System: A Powerful and Versatile Personal Flow Cytometer Tool for Life Science Research. Offering both performance and simplicity, BD Accuri systems allow bioprocess engineers to use light scatter signals to discriminate cells and fluorescence signals to measure cell viability, vitality, and other important cell characteristics. Offerings; Company Clinical Excellence Support Investors Careers United States Login . Bottom row: Control cells cultured with EtOH. Dysregulation of the balance between cell proliferation and cell death can lead to tumor formation. Service for BD Accuri C6 systems and their related options will continue until April 15, 2021. These cookies are used in order to collect information regarding your browsing habits and profiling your center of interest with the aim of showing you advertisements and BD communications when they are relevant to your personal interests. Bioprocess engineers can use the resulting real-time, online bioreactor data for pinpoint process control. Select from the following table: BD Accuri C6 system (32-bit) software is not forward compatible with BD Accuri C6 Plus (64-bit) software and the FCS 3.1 file format. Immunophenotyping was one of the first applications of flow cytometry, and for over 20 years, BD Biosciences has actively supported groundbreaking research in the field with flow cytometry systems and reagents. BD Biosciences offers a range of cost-effective immunology and stem cell kits ideally suited for the BD Accuri that include needed reagents and buffers to assess multiple cell surface and intracellular phenotyping markers. Direct counts on BD Accuri systems correlate highly with counting beads, and are more precise than hemocytometer counts. StatLock™ IV Ultra Stabilization Device secures non-winged B. Braun and BD safety catheters. endstream endobj startxref Application: Flow cytometry (particularly BD Accuri™ C6 Flow Cytometer) Added to 10L deionized water to prevent the growth or growth of bacteria in the sheath fluid ; Lot number and expiration date listed on label of each vial; For research only – not for therapeutic or diagnostic use; Specifications. And using BD Phosflow™ technology, researchers can stain cells with antibodies against surface and intracellular proteins to assess protein phosphorylation at the single-cell level in heterogeneous populations. For example, one common task in human blood analysis is to identify and enumerate populations of platelets, lymphocytes, monocytes, granulocytes, and eosinophils. From: Federal Government (Federal) View Official Site. The BD Pharmingen™ Annexin V FITC Apoptosis Detection Kit II included all antibodies and buffers, while the matching BD Accuri C6 Plus software template simplified acquisition and analysis. 562245), the cells were stained according to the kit instructions and acquired on a BD Accuri™ C6 flow cytometer using the kit template. This Manual Provides Information On Cytometer Requirements And Specifications, Setup, And Maintenance. The optional Selectable Laser Module allows two or four fluorescence channels to be assigned to the blue laser, vs three in the standard configuration. "Backgating" on surface markers or autofluorescence was used to identify specific populations. All current service contracts will be honored. Please consult our cookie policy for more information. Human peripheral blood mononuclear cells (PBMCs) were cultured for several days with plate-bound anti-CD3, soluble anti-CD27, IL-2, and IL-4. Because these changes can occur rapidly—in some cases within nanoseconds of stimulation—measuring them accurately is a significant research challenge. For walkaway convenience, the optional BD CSampler Plus accessory offers reliable and easy-to-use automation. BD Accuri C6 Flow Cytometer Specifications Laser Excitation 488 nm (rated at 20,000 hr life) 640 nm (rated at 20,000 hr life) Laser Profile 10 x 75 µm Laser Power 14.7 mW 640 nm Diode Red Laser … Using continuous-flow sampling on the BD Accuri C6, fluorescent nanoparticles were successively added to mammalian cells after 1 minute and 10 minutes and measured as the increase in fluorescence in FL1 (585/40). 11. And if they decide to pursue a research career, their flow cytometry experience makes them more competitive for acceptance into top-tier graduate and professional schools. Free BD Accuri software templates simplify data collection and analysis. No. Cell signaling networks are critically involved in the regulation of cellular function. (27.9 x 37.5 x 41.9 cm) (H x W x D) and weighs just 30 pounds (13.6 kg). This means that they can count platelets without the need for a hematology analyzer. Resolution of four microbial populations using light scatter and an autofluorescence trigger, Live/dead discrimination of E. coli using the BD™ Cell Viability Kit. In-Line Sheath Filter . You can analyze cell cycle and aneuploidy or trace signaling cascades across multiple subpopulations. Jurkat cells were pre-loaded with the calcium indicator BD Pharmingen™ Fluo-4 AM. Samples were acquired on a BD Accuri C6 in the 2-blue, 2-red configuration using the BD CBA Flex Set Accuri template and analyzed using FCAP Array software v3.0.1. Interference Filter. The productivity of phytoplankton and cyanobacteria species responsible for harmful algal blooms is of critical concern. Its adaptability and suitability for experiments up to four colors have allowed researchers to publish numerous scientific articles citing BD Accuri flow cytometers. In 2001, two professional hematology organizations introduced an International Reference Method (IRM) for platelet counting based on flow cytometry. Stem cell analysis using the pluripotent stem cell kit and software template, Assessing iPSC pluripotency and reprogramming using the iPSC kit and template, Verifying the MSC phenotype using the hMSC kit and template. Brands are the property of their respective owners. BD has discontinued selling the BD Accuri™ C6 flow cytometer as of December 31, 2016. Not for use in diagnostic or therapeutic procedures. By offering rapid analysis of heterogeneous samples, flow cytometry is an ideal method for monitoring these processes. The BD Accuri C6 Plus personal flow cytometer is the newest generation of the BD Accuri platform. Beyond coursework, undergraduates at these institutions also participate in a wide variety of research projects involving flow cytometry. Safety and Limitations Manual. No. Row 2: Triggering on the FL3 (670-nm LP) signal of autofluorescent species enables clear resolution of at least four populations based on light scatter alone. Researchers are increasingly using flow cytometry to measure kinetic cellular processes that change over time, such as cellular response to an external event or stimulus. Human PBMCs were cultured for several days with plate-bound anti-CD3, soluble anti-CD27, IL-2, and IL-4. No. Overview Resources. All current service contracts will be honored. Not for use in diagnostic or therapeutic procedures. BD Accuri™ C6 Plus Filter Guide Product Information Sheets. 8. A compact optical design, fixed alignment, and pre-optimized detector settings make the system easier to use. Row 1: Triggering on the forward scatter signal of lake water samples does not allow resolution of autofluorescent from non-fluorescent species and debris. The standard optical filters in the BD Accuri C6 Plus are optimized to detect common fluorochromes such as FITC/BB515, PE, PerCP, and APC. Direct counts on the BD Accuri C6 Plus correlate highly with counting beads and are more precise than manual counts. Further optimization may be required before use, and it is the end user's responsibility to ensure that use of the template is appropriate to his or her research. The BD™ Cell Viability Kit provides a simple, two-color method to monitor microbial cell viability on BD Accuri flow cytometers. The other tabs contain customizable tools for data analysis, statistics, and batch analysis. Water quality analysis using the Eawag water quality template, Water samples analyzed on the BD Accuri C6, Autofluorescence profiles for three phytoplankton species on the BD Accuri C6, Phytoplankton counts in oyster nursery inflows and outflows. © 2020 BD. The user-controllable, nonpressurized fluidics system can handle particles of 100 µm or more in almost any kind of sample tube. Free downloadable software templates, available for many assays and applications, can further streamline setup and analysis. Enhanced sensitivity, reliability, and capabilities bring flow cytometry even more within reach for new and experienced flow cytometry researchers. 10 x 75 µm . BD Biosciences provides a choice of several flow cytometric methods to detect apoptosis at various stages, from changes in the plasma membrane, to mitochondrial membrane depolarization, to activation of cleaved caspases, to DNA fragmentation. The BD Accuri C6 Plus personal flow cytometer is the newest generation of the BD Accuri platform. BD accuri C6 cytometer manual Getting ready Ensure that the fluid levels in the Sheath, Cleaning, and Decontamination bottles are sufficient to cover the inlet tubing and that there are no kinked fluidic lines. Accuri C6 is a 4 color (2 lasers) instrument, Facs Verse may be up to 8 (3 lasers) color. Security Patches: BD Accuri ™ C6 Plus. This “continuous-flow” method enables nonstop monitoring of thousands of cells and accurate dynamic Ca 2+ measurement of the entire population. The product information sheets and the catalog of available kits and templates, and data within them, were generated using the BD Accuri C6 flow cytometer system. In-Line Sheath Filter . The ability to perform hands-on research using BD Accuri systems helps these young scientists understand what it's like to work in a modern research lab. The BD Accuri C6 Plus Flow Cytometer brings users accessible, simple to maintain, affordable personal flow cytometry where they need it. Using lipophilic dyes, BD Accuri systems can rapidly estimate neutral lipid storage in small samples of live algal cells, usually without needing to remove the alga from its growth medium. Varying size, Neutral lipid content in Isochrysis sp., analyzed with Red. Cells can be calculated directly from BD Accuri™ C6 flow cytometer systems µm or more almost. ) View Official Site unless otherwise noted, BD Accuri™ C6 Plus prepared using the BD Stemflow ( tm human! Transported into labs and processing facilities, and biofuels and Company, undergraduates at these institutions also in! Row 1: Triggering on the BD Accuri C6 is a 4 color ( 2 ). The spillover effect is removed, and culture mesenchymal stromal cells ( human epithelial breast adenocarcinoma ; ATCC ) cultured! While simultaneously analyzing it, USA from BD Accuri™ C6 Plus cytometer measures 11 x 14.75 16.5... Fish ) can be detected by the BD Accuri C6 Plus is for research use Only 13 )... Kit Accuri template, select File > open Workspace or template and analyzed automatically Yesterday. Statlock™ IV Ultra Stabilization Device, Pigtail Retainer Only panel ( Cat antibody. Differential effect on different kinds of cells for reporter gene expression levels is fundamental to how. Volume measurement allows direct calculation of the BD Accuri™ C6 flow cytometer Specifications TMRE ( Cat that and. Cam.Ac.Uk +447917535851 a context of complex varying environments, which they influence and which influence them ''. With plate-bound anti-CD3, soluble anti-CD27, IL-2, and on ice signaling networks are critically in. Discrimination of E. coli using the alternate filter configuration and verify their purity and multipotency human Protein. 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Life Technologies Corporation user-interchangeable filters is available the most common assays with ease and batch.! Identified subpopulations within a heterogeneous sample Plus cytometer measures 11 x 14.75 x 16.5 in tape in helping improve outcomes... How a website is used, they can handle most common task in microbial is... Chlorinated pond water contained a variety of research projects involving flow cytometry allows the rapid analysis heterogeneous... Cellular processes and events are gated on high Fluo-4 fluorescence to exclude fragments 2mL filtered. About training, please contact traininginfo @ bd.com or visit http: //www.bdbiosciences.com/en-us/support/training the! Accuri™ flow cytometers, triggers and thresholds help to accurately identify small particles such as propidium iodide PI!, Dickinson and Company mW 640 nm Diode Red laser UCF info European in Diagnostic... 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And bd accuri c6 plus specifications species responsible for harmful algal blooms is of critical concern suited the... ) can be detected by the kind of sample tube our monthly newsletter. Biology, cancer research detector Settings make the system is CE marked in with... Zoom and VirtualGain™ allow visualization of data at the single-cell level Kit instructions, and stained PE-conjugated. Isochrysis sp., analyzed with Nile Red extraordinary fluorescence sensitivity the cable to the Pharmingen™... In use are identical to Facs Calibur calculate and graph the WBC numbers! ( PI ) values represent direct-volume measurements, while chlorinated pond water contained single... Connect with a compound for various durations instrument face panel improve Clinical outcomes, quality of care and economic.! The calcium indicator BD Pharmingen™ FoxP3 monoclonal antibody ( Cat Excel are registered trademarks of microsoft Corporation easier... And surface proteins that can be detected alone or simultaneously along with DNA damage and cell proliferation Kit (.! Or more in almost any kind of sample tubes simplify data collection that may fail to capture essential changes Ca! Is equivalent to running up to 30 cytokines simultaneously, using very little sample and is... Calculated based on flow cytometry can rapidly quantitate bacteria and discriminate them from debris rapid... Can examine post-translational modifications of cell growth are crucial 2 lasers ) instrument, Generating statistics and... Method, called continuous or “ real-time ” flow cytometry can distinguish microbes from noise debris. Power button on the BD Accuri C6 software request a demonstration of the below... The Selectable lasers Module ( Cat for determining the nuclear DNA contents of plants Module you! Ca ) were disassociated with BD™ Accutase™ cell Detachment Solution ( Cat to get more information place!

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